AT5G49660 : XYLEM INTERMIXED WITH PHLOEM 1
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AGICode AT5G49660
Description Leucine-rich repeat transmembrane protein kinase family protein
Gene information
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  Gene Code Description / Information Gene name Correlation link
pcc 2.5% 97.5% PPI
1 AT5G49660 Leucine-rich repeat transmembrane protein kinase family
protein
XYLEM INTERMIXED WITH PHLOEM 1 1 0.31 -0.31
2 AT4G05400 copper ion binding -0.86 0.32 -0.33
3 AT2G33370 Ribosomal protein L14p/L23e family protein -0.84 0.32 -0.31
4 AT1G18470 Transmembrane Fragile-X-F-associated protein 0.83 0.32 -0.33
5 AT2G25625 unknown protein; FUNCTIONS IN: molecular_function unknown;
INVOLVED IN: biological_process unknown; LOCATED IN:
chloroplast; EXPRESSED IN: 10 plant structures; EXPRESSED
DURING: LP.06 six leaves visible, LP.04 four leaves
visible, 4 anthesis, petal differentiation and expansion
stage; Has 24 Blast hits to 24 proteins in 9 species:
Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants -
24; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
0.82 0.31 -0.32
6 AT1G24280 glucose-6-phosphate dehydrogenase 3 glucose-6-phosphate dehydrogenase
3
-0.82 0.33 -0.33
7 AT1G17550 homology to ABI2 homology to ABI2 0.82 0.31 -0.31
8 AT1G62310 transcription factor jumonji (jmjC) domain-containing
protein
0.81 0.31 -0.32
9 AT5G13200 GRAM domain family protein 0.81 0.32 -0.3
10 AT4G25740 RNA binding Plectin/S10 domain-containing protein -0.81 0.32 -0.31
11 AT4G25890 60S acidic ribosomal protein family -0.8 0.32 -0.33
12 AT2G38790 unknown protein; Has 21 Blast hits to 21 proteins in 5
species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0;
Plants - 19; Viruses - 0; Other Eukaryotes - 2 (source:
NCBI BLink).
0.8 0.3 -0.32
13 AT2G40460 Major facilitator superfamily protein 0.8 0.3 -0.3
14 AT5G67510 Translation protein SH3-like family protein -0.8 0.32 -0.3
15 AT3G01560 Protein of unknown function (DUF1421) 0.8 0.34 -0.3
16 AT3G53960 Major facilitator superfamily protein 0.79 0.3 -0.33
17 AT2G28660 Chloroplast-targeted copper chaperone protein -0.79 0.33 -0.32
18 AT3G04770 40s ribosomal protein SA B 40s ribosomal protein SA B -0.79 0.32 -0.31
19 AT3G07360 plant U-box 9 ARABIDOPSIS THALIANA PLANT U-BOX
9, plant U-box 9
0.79 0.35 -0.32
20 AT1G52300 Zinc-binding ribosomal protein family protein -0.79 0.33 -0.3
21 AT1G72370 40s ribosomal protein SA AP40, 40s ribosomal protein SA,
RP40, RPSAA
-0.79 0.33 -0.32
22 AT2G45730 eukaryotic initiation factor 3 gamma subunit family protein -0.79 0.3 -0.33
23 AT2G47790 Transducin/WD40 repeat-like superfamily protein -0.79 0.32 -0.35
24 AT1G27200 Domain of unknown function (DUF23) 0.79 0.3 -0.31
25 AT3G11250 Ribosomal protein L10 family protein -0.78 0.31 -0.31
26 AT3G28007 Nodulin MtN3 family protein AtSWEET4, SWEET4 0.78 0.32 -0.32
27 AT2G40660 Nucleic acid-binding, OB-fold-like protein -0.78 0.31 -0.31
28 AT3G52580 Ribosomal protein S11 family protein -0.78 0.31 -0.34
29 AT2G35605 SWIB/MDM2 domain superfamily protein -0.78 0.32 -0.3
30 AT5G01820 serine/threonine protein kinase 1 ATCIPK14, serine/threonine protein
kinase 1, CBL-INTERACTING PROTEIN
KINASE 14, SOS2-like protein
kinase 24, SNF1-RELATED PROTEIN
KINASE 3.15, serine/threonine
protein kinase 1
0.78 0.31 -0.31
31 AT5G15520 Ribosomal protein S19e family protein -0.77 0.32 -0.34
32 AT1G15930 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family protein -0.77 0.32 -0.29
33 AT5G62000 auxin response factor 2 ARF1-BINDING PROTEIN, auxin
response factor 2, HLS1
SUPPRESSOR, ORESARA 14
0.77 0.3 -0.3
34 AT1G31480 shoot gravitropism 2 (SGR2) SHOOT GRAVITROPISM 2 0.77 0.31 -0.32
35 AT2G36170 Ubiquitin supergroup;Ribosomal protein L40e -0.77 0.31 -0.31
36 AT5G35530 Ribosomal protein S3 family protein -0.77 0.34 -0.31
37 AT4G30790 INVOLVED IN: autophagy; EXPRESSED IN: 24 plant structures;
EXPRESSED DURING: 15 growth stages; CONTAINS InterPro
DOMAIN/s: Autophagy-related protein 17
(InterPro:IPR007240), Autophagy-related protein 11
(InterPro:IPR019460); Has 8793 Blast hits to 6268 proteins
in 570 species: Archae - 89; Bacteria - 657; Metazoa -
4844; Fungi - 808; Plants - 441; Viruses - 15; Other
Eukaryotes - 1939 (source: NCBI BLink).
0.77 0.34 -0.32
38 AT2G22430 homeobox protein 6 homeobox protein 6, homeobox
protein 6
0.77 0.31 -0.32
39 AT3G44590 60S acidic ribosomal protein family -0.77 0.32 -0.31
40 AT1G07370 proliferating cellular nuclear antigen 1 PROLIFERATING CELLULAR NUCLEAR
ANTIGEN 1, proliferating cellular
nuclear antigen 1
-0.77 0.33 -0.32
41 AT1G29250 Alba DNA/RNA-binding protein -0.76 0.3 -0.34
42 AT3G58700 Ribosomal L5P family protein -0.76 0.31 -0.3
43 AT1G29980 Protein of unknown function, DUF642 -0.76 0.3 -0.31
44 AT3G20300 Protein of unknown function (DUF3537) 0.76 0.32 -0.33
45 AT1G26910 Ribosomal protein L16p/L10e family protein ribosomal protein L10 B -0.76 0.3 -0.33
46 AT2G22010 related to KPC1 related to KPC1 0.75 0.32 -0.31
47 AT3G07270 GTP cyclohydrolase I -0.75 0.32 -0.31
48 AT1G20880 RNA-binding (RRM/RBD/RNP motifs) family protein 0.75 0.33 -0.31
49 AT5G66920 SKU5 similar 17 SKU5 similar 17 -0.75 0.3 -0.31
50 AT1G24070 cellulose synthase-like A10 cellulose synthase-like A10,
CELLULOSE SYNTHASE LIKE A10,
cellulose synthase-like A10
0.75 0.31 -0.31
51 AT3G07110 Ribosomal protein L13 family protein -0.75 0.28 -0.31
52 AT5G64100 Peroxidase superfamily protein -0.75 0.31 -0.33
53 AT5G16730 Plant protein of unknown function (DUF827) 0.75 0.32 -0.32
54 AT5G44900 Toll-Interleukin-Resistance (TIR) domain family protein -0.74 0.31 -0.32
55 AT1G76590 PLATZ transcription factor family protein 0.74 0.3 -0.32
56 AT4G35790 phospholipase D delta ARABIDOPSIS THALIANA PHOSPHOLIPASE
D DELTA, phospholipase D delta
0.74 0.32 -0.32
57 AT2G14460 unknown protein; Has 3 Blast hits to 3 proteins in 1
species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0;
Plants - 3; Viruses - 0; Other Eukaryotes - 0 (source: NCBI
BLink).
-0.74 0.3 -0.31
58 AT2G32060 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family protein -0.74 0.31 -0.31
59 AT1G54690 gamma histone variant H2AX GAMMA H2AX, gamma histone variant
H2AX, H2AXB, histone H2A 3
-0.74 0.32 -0.31
60 AT1G47290 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 3beta-hydroxysteroid-dehydrogenase
/decarboxylase isoform 1,
3beta-hydroxysteroid-dehydrogenase
/decarboxylase isoform 1
-0.74 0.3 -0.33
61 AT3G14050 RELA/SPOT homolog 2 RELA-SPOT HOMOLOG 2, RELA/SPOT
HOMOLOG 2, RELA/SPOT homolog 2
0.74 0.29 -0.31
62 AT2G17990 BEST Arabidopsis thaliana protein match is:
kinectin-related (TAIR:AT5G66250.3); Has 7578 Blast hits to
6129 proteins in 783 species: Archae - 220; Bacteria -
1045; Metazoa - 3605; Fungi - 575; Plants - 442; Viruses -
38; Other Eukaryotes - 1653 (source: NCBI BLink).
0.74 0.35 -0.32
63 AT1G61580 R-protein L3 B ARABIDOPSIS RIBOSOMAL PROTEIN 2,
R-protein L3 B
-0.74 0.34 -0.33
64 AT2G32090 Lactoylglutathione lyase / glyoxalase I family protein 0.74 0.28 -0.33
65 AT1G17560 Ribosomal protein L14p/L23e family protein HUELLENLOS -0.74 0.34 -0.31
66 AT1G65580 Endonuclease/exonuclease/phosphatase family protein FRAGILE FIBER3 0.74 0.3 -0.32
67 AT1G54130 RELA/SPOT homolog 3 AT-RSH3, RELA/SPOT HOMOLOG 3,
RELA/SPOT homolog 3
0.74 0.31 -0.3
68 AT1G58360 amino acid permease 1 amino acid permease 1, NEUTRAL
AMINO ACID TRANSPORTER 2
0.74 0.33 -0.32
69 AT1G01100 60S acidic ribosomal protein family -0.74 0.31 -0.33
70 AT1G74560 NAP1-related protein 1 NAP1-related protein 1 -0.74 0.3 -0.3
71 AT1G03080 kinase interacting (KIP1-like) family protein 0.74 0.31 -0.3
72 AT2G39700 expansin A4 ATEXP4, expansin A4, ATHEXP ALPHA
1.6, expansin A4
-0.74 0.32 -0.34
73 AT5G54730 homolog of yeast autophagy 18 (ATG18) F ARABIDOPSIS THALIANA HOMOLOG OF
YEAST AUTOPHAGY 18 (ATG18) F,
homolog of yeast autophagy 18
(ATG18) F, homolog of yeast
autophagy 18 (ATG18) F
0.74 0.3 -0.33
74 AT2G18400 ribosomal protein L6 family protein -0.74 0.3 -0.32
75 AT1G80560 isopropylmalate dehydrogenase 2 ARABIDOPSIS ISOPROPYLMALATE
DEHYDROGENASE 2, isopropylmalate
dehydrogenase 2
-0.73 0.32 -0.32
76 AT1G16920 RAB GTPase homolog A1B RAB GTPase homolog A1B, RAB11, RAB
GTPase homolog A1B
-0.73 0.32 -0.34
77 AT1G15810 S15/NS1, RNA-binding protein -0.73 0.31 -0.31
78 AT4G25870 Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase
family protein
-0.73 0.3 -0.33
79 AT1G04190 Tetratricopeptide repeat (TPR)-like superfamily protein tetratricopeptide repeat 3 -0.73 0.31 -0.31
80 AT3G21270 DOF zinc finger protein 2 DOF zinc finger protein 2, DOF
zinc finger protein 2
0.73 0.33 -0.34
81 AT2G37600 Ribosomal protein L36e family protein -0.73 0.34 -0.3
82 AT1G73480 alpha/beta-Hydrolases superfamily protein 0.73 0.3 -0.33
83 AT5G55260 protein phosphatase X 2 PROTEIN PHOSPHATASE X -2, protein
phosphatase X 2
-0.73 0.32 -0.32
84 AT1G04590 BEST Arabidopsis thaliana protein match is:
Pentatricopeptide repeat (PPR) superfamily protein
(TAIR:AT4G21190.1); Has 111 Blast hits to 111 proteins in
15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi -
0; Plants - 109; Viruses - 0; Other Eukaryotes - 2 (source:
NCBI BLink).
-0.73 0.33 -0.31
85 AT3G25540 TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing
protein
LONGEVITY ASSURANCE GENE 1 -0.73 0.32 -0.3
86 AT2G37190 Ribosomal protein L11 family protein -0.73 0.34 -0.3
87 AT3G53020 Ribosomal protein L24e family protein RIBOSOMAL PROTEIN L24, RPL24B,
SHORT VALVE1
-0.73 0.31 -0.31
88 AT5G50810 translocase inner membrane subunit 8 translocase inner membrane subunit
8
-0.73 0.31 -0.34
89 AT3G12390 Nascent polypeptide-associated complex (NAC), alpha subunit
family protein
-0.73 0.35 -0.32
90 AT5G02530 RNA-binding (RRM/RBD/RNP motifs) family protein -0.73 0.3 -0.31
91 AT3G02080 Ribosomal protein S19e family protein -0.73 0.31 -0.32
92 AT3G55010 phosphoribosylformylglycinamidine cyclo-ligase, chloroplast
/ phosphoribosyl-aminoimidazole synthetase / AIR synthase
(PUR5)
ATPURM, EMBRYO DEFECTIVE 2818,
PUR5
-0.73 0.31 -0.32
93 AT2G39460 ribosomal protein L23AA ARABIDOPSIS THALIANA RIBOSOMAL
PROTEIN L23A, RIBOSOMAL PROTEIN
L23A, RIBOSOMAL PROTEIN L23A1,
ribosomal protein L23AA
-0.73 0.3 -0.31
94 AT3G29670 HXXXD-type acyl-transferase family protein phenolic glucoside
malonyltransferase 2
0.73 0.32 -0.32
95 AT1G70310 spermidine synthase 2 spermidine synthase 2 -0.73 0.3 -0.32
96 AT3G16940 calmodulin binding;transcription regulators 0.72 0.3 -0.34
97 AT1G02780 Ribosomal protein L19e family protein embryo defective 2386 -0.72 0.31 -0.33
98 AT4G18800 RAB GTPase homolog A1D ATHSGBP, ATRAB11B, RAB GTPase
homolog A1D, RAB GTPase homolog
A1D
-0.72 0.32 -0.3
99 AT3G62370 heme binding -0.72 0.31 -0.35
100 AT1G56650 production of anthocyanin pigment 1 MYB DOMAIN PROTEIN 75,
MYELOBLASTOSIS PROTEIN 75,
production of anthocyanin pigment
1, SUC-INDUCED ANTHOCYANIN
ACCUMULATION 1
0.72 0.32 -0.3
101 AT3G06610 DNA-binding enhancer protein-related -0.72 0.32 -0.33
102 AT5G15830 basic leucine-zipper 3 basic leucine-zipper 3, basic
leucine-zipper 3
-0.72 0.33 -0.31
103 AT1G59910 Actin-binding FH2 (formin homology 2) family protein 0.72 0.33 -0.31
104 AT5G39740 ribosomal protein L5 B OLIGOCELLULA 7, ribosomal protein
L5 B
-0.72 0.33 -0.33
105 AT5G18640 alpha/beta-Hydrolases superfamily protein 0.72 0.29 -0.32
106 AT3G18040 MAP kinase 9 MAP kinase 9 0.72 0.31 -0.32
107 AT5G58670 phospholipase C1 ARABIDOPSIS THALIANA PHOSPHOLIPASE
C, phospholipase C1, phospholipase
C 1, phospholipase C1
0.72 0.32 -0.32
108 AT1G50490 ubiquitin-conjugating enzyme 20 ubiquitin-conjugating enzyme 20 -0.72 0.35 -0.32
109 AT5G10360 Ribosomal protein S6e embryo defective 3010, Ribosomal
protein small subunit 6b
-0.72 0.32 -0.32
110 AT5G35560 DENN (AEX-3) domain-containing protein 0.72 0.33 -0.33
111 AT2G19730 Ribosomal L28e protein family -0.72 0.31 -0.32
112 AT1G06790 RNA polymerase Rpb7 N-terminal domain-containing protein -0.72 0.29 -0.33
113 AT5G23740 ribosomal protein S11-beta ribosomal protein S11-beta -0.72 0.32 -0.33
114 AT5G60140 AP2/B3-like transcriptional factor family protein -0.72 0.3 -0.31
115 AT3G09630 Ribosomal protein L4/L1 family -0.72 0.31 -0.32
116 AT4G31460 Ribosomal L28 family -0.72 0.33 -0.31
117 AT3G06930 protein arginine methyltransferase 4B ARABIDOPSIS THALIANA PROTEIN
ARGININE METHYLTRANSFERASE 4B,
protein arginine methyltransferase
4B
-0.71 0.32 -0.29
118 AT2G26890 DNAJ heat shock N-terminal domain-containing protein GRAVITROPISM DEFECTIVE 2,
KATAMARI2
0.71 0.32 -0.3
119 AT5G47700 60S acidic ribosomal protein family -0.71 0.31 -0.32
120 AT1G59510 Carbohydrate-binding protein CF9 0.71 0.33 -0.3
121 AT1G68570 Major facilitator superfamily protein 0.71 0.31 -0.33
122 AT1G67430 Ribosomal protein L22p/L17e family protein -0.71 0.33 -0.31
123 AT3G25790 myb-like transcription factor family protein -0.71 0.31 -0.3
124 AT3G44750 histone deacetylase 3 ATHD2A, HISTONE DEACETYLASE 2A,
histone deacetylase 3, HDT1
-0.71 0.32 -0.32
125 AT5G56940 Ribosomal protein S16 family protein -0.71 0.33 -0.32
126 AT1G76300 snRNP core protein SMD3 snRNP core protein SMD3 -0.71 0.31 -0.31
127 AT1G21690 ATPase family associated with various cellular activities
(AAA)
embryo defective 1968, replication
factor C 4
-0.71 0.31 -0.32
128 AT1G65890 acyl activating enzyme 12 acyl activating enzyme 12 0.71 0.31 -0.3
129 AT5G16070 TCP-1/cpn60 chaperonin family protein -0.71 0.32 -0.32
130 AT4G33040 Thioredoxin superfamily protein 0.71 0.3 -0.32
131 AT3G07580 unknown protein; FUNCTIONS IN: molecular_function unknown;
INVOLVED IN: biological_process unknown; LOCATED IN:
chloroplast; EXPRESSED IN: 18 plant structures; EXPRESSED
DURING: 12 growth stages; BEST Arabidopsis thaliana protein
match is: unknown protein (TAIR:AT5G48335.1); Has 30201
Blast hits to 17322 proteins in 780 species: Archae - 12;
Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants -
5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI
BLink).
-0.71 0.31 -0.3
132 AT3G61140 26S proteasome, regulatory subunit Rpn7;Proteasome
component (PCI) domain
ARABIDOPSIS THALIANA FUSCA 6,
SHAGGY-LIKE KINASE 31,
CONSTITUTIVE PHOTOMORPHOGENIC 11,
COP9 SIGNALOSOME SUBUNIT 1, EMBRYO
DEFECTIVE 78, FUSCA 6, SHAGGY-LIKE
KINASE 31
-0.71 0.35 -0.31
133 AT2G40435 BEST Arabidopsis thaliana protein match is: transcription
regulators (TAIR:AT3G56220.1); Has 289 Blast hits to 289
proteins in 30 species: Archae - 0; Bacteria - 0; Metazoa -
0; Fungi - 0; Plants - 289; Viruses - 0; Other Eukaryotes -
0 (source: NCBI BLink).
0.71 0.29 -0.31
134 AT2G29570 proliferating cell nuclear antigen 2 A. THALIANA PROLIFERATING CELL
NUCLEAR ANTIGEN 2, proliferating
cell nuclear antigen 2
-0.71 0.33 -0.31
135 AT1G55110 indeterminate(ID)-domain 7 indeterminate(ID)-domain 7,
indeterminate(ID)-domain 7
0.7 0.31 -0.31
136 ATCG00065 ribosomal protein S12A RIBOSOMAL PROTEIN S12, ribosomal
protein S12A
0.7 0.31 -0.32
137 AT3G52820 purple acid phosphatase 22 PURPLE ACID PHOSPHATASE 22, purple
acid phosphatase 22
0.7 0.32 -0.33
138 AT3G03870 unknown protein; BEST Arabidopsis thaliana protein match
is: unknown protein (TAIR:AT5G18130.1); Has 41 Blast hits
to 41 proteins in 11 species: Archae - 0; Bacteria - 0;
Metazoa - 2; Fungi - 0; Plants - 39; Viruses - 0; Other
Eukaryotes - 0 (source: NCBI BLink).
0.7 0.31 -0.32
139 AT1G35660 unknown protein; FUNCTIONS IN: molecular_function unknown;
INVOLVED IN: biological_process unknown; LOCATED IN: plasma
membrane; EXPRESSED IN: 19 plant structures; EXPRESSED
DURING: 11 growth stages; Has 309 Blast hits to 256
proteins in 99 species: Archae - 0; Bacteria - 11; Metazoa
- 192; Fungi - 12; Plants - 36; Viruses - 0; Other
Eukaryotes - 58 (source: NCBI BLink).
0.7 0.31 -0.34
140 AT5G05570 transducin family protein / WD-40 repeat family protein 0.69 0.31 -0.31
141 AT5G48370 Thioesterase/thiol ester dehydrase-isomerase superfamily
protein
0.69 0.33 -0.31
142 AT1G70750 Protein of unknown function, DUF593 0.69 0.32 -0.32
143 AT5G57390 AINTEGUMENTA-like 5 AINTEGUMENTA-like 5, CHOTTO 1,
EMBRYOMAKER, PLETHORA 5
0.69 0.34 -0.31
144 AT3G15880 WUS-interacting protein 2 TOPLESS-RELATED 4, WUS-interacting
protein 2
0.69 0.33 -0.33
145 AT3G14440 nine-cis-epoxycarotenoid dioxygenase 3 ATNCED3, nine-cis-epoxycarotenoid
dioxygenase 3, SUGAR INSENSITIVE
7, SALT TOLERANT 1
0.69 0.34 -0.32
146 AT5G52660 Homeodomain-like superfamily protein 0.69 0.32 -0.34
147 AT2G04880 zinc-dependent activator protein-1 ATWRKY1, WRKY1, zinc-dependent
activator protein-1
0.68 0.31 -0.3
148 AT5G12140 cystatin-1 cystatin-1, cystatin-1 0.68 0.33 -0.33
149 AT5G13640 phospholipid:diacylglycerol acyltransferase ARABIDOPSIS THALIANA
PHOSPHOLIPID:DIACYLGLYCEROL
ACYLTRANSFERASE,
phospholipid:diacylglycerol
acyltransferase,
PHOSPHOLIPID:DIACYLGLYCEROL
ACYLTRANSFERASE 1
0.68 0.31 -0.29
150 AT2G15320 Leucine-rich repeat (LRR) family protein 0.68 0.33 -0.32
151 AT2G38410 ENTH/VHS/GAT family protein 0.68 0.31 -0.31
152 AT1G76360 Protein kinase superfamily protein 0.68 0.29 -0.31
153 AT2G23760 BEL1-like homeodomain 4 BEL1-like homeodomain 4, SAWTOOTH
2
0.68 0.33 -0.33
Metabolite information
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  CID Metabolite name Pathway Information Correlation link
Compound name Stereochemistry Aracyc name pcc 2.5% 97.5% PPI
154 C0258 Tryptophan amide (NH3+) D,L-Tryptophan amide (NH3+) - - 0.78 0.45 -0.4
155 C0241 Stigmasterol 3-O-β-D-glucoside - Stigmasterol 3-O-β-D-glucoside - 0.72 0.47 -0.51 C0241
156 C0257 Tryptophan L-Tryptophan L-Tryptophan glucosinolate biosynthesis from tryptophan,
IAA biosynthesis I,
tryptophan biosynthesis,
camalexin biosynthesis,
tRNA charging
0.68 0.31 -0.3 C0257